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Features
Designed for the clinical diagnostics lab
Simple and fast
Ready to use in 10 minutes with an all-in-one cartridge that integrates reagents directly into the microfluidic chip. Same-day results in as little as 5 hours — no complex setup, no bottlenecks from third-party service providers.
High accuracy, no bias
DNBSEQ™ technology produces DNA Nanoballs via rolling circle replication — no PCR, no amplification errors. Delivers 90% fewer duplicates, reduced index hopping, and >90% Q30 data quality versus traditional sequencing platforms.
Flexible and secure
25 million reads per run — perfectly sized for targeted panels, amplicon sequencing, and small-to-medium studies. Operates fully standalone with no cloud dependency. Patient data stays entirely in-house, ensuring regulatory compliance and data sovereignty.
Technology
Powered by DNBSEQ™ technology
A fundamentally different approach to sequencing — four steps that eliminate PCR errors, index hopping, and the need for external optical systems.
Step 1 of 4
DNA circularisation
Unlike Illumina which keeps DNA as linear fragments, DNBSEQ™ converts each DNA fragment into a stable circular template first. Double-stranded DNA is denatured into single strands. A splint oligonucleotide bridges the two adapter ends, and DNA ligase seals the nick — creating a complete single-stranded circle (ssCirDNA).
This circular template is stable against exonuclease degradation and enables rolling circle replication in the next step — the key reason DNBSEQ™ avoids PCR entirely.
DNBSEQ™
Circular ssDNA — stable, no end degradation, PCR-free
Illumina
Linear dsDNA — bridge PCR required, amplification errors possible
Applications
Key applications for abSEQ™
The 25M read throughput is ideally suited for targeted panels and small-to-medium studies across a wide range of genomic applications.
Amplicon-based targeted sequencing
1–250 samples
Enrichment-based targeted sequencing
1–250 samples
Genome editing
1–250 samples
Immune repertoire sequencing
1–12 samples
3' gene expression
5–25 samples
Targeted RNA panel
5–25 samples
Pathogen detection
1–25 samples
16S amplicon sequencing
1–250 samples
Shallow shotgun metagenomics
1–12 samples
Shotgun metagenomics
1–2 samples
Small whole genome sequencing
1–25 samples
Sequencing Sets
abSEQ™ Sequencing Sets
Four sequencing sets covering single-end and paired-end runs, with and without the App-C invertase module.
* Effective reads based on operation of a specific standard library. Actual values may vary by sample type and library construction method. ** Q30 and run time averaged over entire run; actual performance may vary by sample type, library quality, and insert length. *** App-C Sequencing Set includes invertase module — eliminates the need to purchase additional conversion reagents.
Ready to bring sequencing in-house?
Contact our team to arrange a demonstration, discuss application fit, or request a quotation for abSEQ™ and compatible sequencing sets.